Humanization of recombinant glycoproteins expressed in insect cells
نویسندگان
چکیده
منابع مشابه
recombinant outer capsid glycoprotein (vp7) of rotavirus expressed in insect cells induces neutralizing antibodies in rabbits
background: rotaviruses cause diarrhea in infants and young children worldwide. rotavirus outer capsid protein, vp7 is major neutralizing antigen that is important component of subunit vaccine to prevent rotavirus infection. many efforts have been done to produce recombinant vp7 that maintain native characteristics. we used baculovirus expression system to produce rotavirus vp7 protein and to s...
متن کاملCharacterization of recombinant E-cadherin (uvomorulin) expressed in insect cells.
Cadherins are Ca(2+)-dependent cell adhesion molecules that mediate cell adhesion by homophilic binding. Structural and functional analysis of the extracellular part of cadherins that mediates this binding has often been hampered by the availability of sufficient amount of protein. Therefore, we have expressed the extracellular region of E-cadherin (uvomorulin) using the baculovirus expression ...
متن کاملNovel baculovirus expression vectors that provide sialylation of recombinant glycoproteins in lepidopteran insect cells.
This report describes novel baculovirus vectors designed to express mammalian beta1,4-galactosyltransferase and alpha2,6-sialyltransferase genes at early times after infection. Sf9 cells infected with these viral vectors, unlike cells infected with a wild-type baculovirus, produced a sialylated viral glycoprotein during the late phase of infection. Thus, the two mammalian glycosyltransferases e...
متن کاملassessment of selective toxicity of insect cell expressed recombinant a1-gmcsf protein toward gmcsf receptor bearing tumor cells
one of the emerging therapeutic strategies for targeted treatment of most cancers is the use of immunotoxins which are fusion proteins consisted of a targeting and a toxic moieties. we previously showed that the recombinant a254-gmcsf fusion protein selectively kills acute myeloblastic leukemia cells which harbor a large number of granulocyte-macrophage colony stimulating factor (gmcsf) recepto...
متن کاملRecombinant Outer Capsid Glycoprotein (VP7) of Rotavirus Expressed in Insect Cells Induces Neutralizing Antibodies in Rabbits
BACKGROUND Rotaviruses cause diarrhea in infants and young children worldwide. Rotavirus outer capsid protein, VP7 is major neutralizing antigen that is important component of subunit vaccine to prevent rotavirus infection. Many efforts have been done to produce recombinant VP7 that maintain native characteristics. We used baculovirus expression system to produce rotavirus VP7 protein and to st...
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ژورنال
عنوان ژورنال: Trends in Glycoscience and Glycotechnology
سال: 2009
ISSN: 0915-7352,1883-2113
DOI: 10.4052/tigg.21.71